High-precision breeding: reliable and cost-efficient molecular tools are looking for users
2008
Winter, P., GenXPro GmbH, Frankfurt (Germany)
Recent advances in all areas of molecular breeding as e.g. comparative genomic, or SuperSAGE whole-genome transcription profiling provide unprecedented insights into molecular components underlying traits in non-model crops. Novel tools based on these insights such as custom gene-expression and SNP-detection micro-arrays, and qRT-PCR assays to detect SNPs and differential expression of genes in response to development signals and in response to the environment have been developed also in legumes and are available to everybody. We will give an overview of progress in understanding the biotic stress responses of major European legumes to fungal pathogens and demonstrate the power of SuperSAGE for the identification of potential breeding targets for dehydration stress tolerance in chickpea. Downstream of SuperSAGE, fast, reliable and cost-efficient qRT-PCR assay a indicative for stress-response pathways offer themselves for application in knowledge-based breeding. Especially, a molecular definition of trait components available in hitherto under-exploited wild and cultivated germplasm that could be introgressed with as yet unprecedented speed and accuracy could pave the way to new varieties better suited to changing market needs. Now, intense communication between molecular biologists, plant physiologists and breeders is required to drive molecular approaches into application-oriented directions. This presentation is meant to elicit such communication.
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