Bulk segregation analysis and improvement of seed quality in soybean
2008
Đorđević, V., Institute of Field and Vegetable Crops, Novi Sad (Serbia) | Miladinović, J., Institute of Field and Vegetable Crops, Novi Sad (Serbia) | Balešević-Tubić, S., Institute of Field and Vegetable Crops, Novi Sad (Serbia) | Đukić, V., Institute of Field and Vegetable Crops, Novi Sad (Serbia) | Mikić, A., Institute of Field and Vegetable Crops, Novi Sad (Serbia)
One of the goals in soybean breeding program is increasing protein content. On LG I was located major QTL for protein content, originate from Glycine soja. This protein QTL was detected in some Glycine max genotypes, mostly non-adapted germplasm which are inadequate for commercial breeding programs. Goal of this work is to find suitable source for marker assisted selection of soybean to increase seed protein content. A total of 155 F2 plants of soybean, were developed from a cross of Senka x Novosadjanka used in this study. Base on a previous work, can suppose variety Senka is donor parent of protein QTL from LG I. Bulk segregation analysis was conducted base on protein content and tested for differences in allele frequency between the population bulks with microsatellite marker Satt239. This marker is closely linked with protein QTL from Glycine soja. Each bulk contains 12 high protein and 12 low protein F2 soybean lines. These results show potential of molecular markers in soybean breeding programs. Future research will show reaction of this QTL in different genetic backgrounds and environments and usefulness in soybean breeding.
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