Incidence of viruses in the most important bean varieties in Vojvodina [Serbia]
2008
Petrović, D., Institute of Field and Vegetable Crops, Novi Sad (Serbia) | Ignjatov, M., Institute of Field and Vegetable Crops, Novi Sad (Serbia) | Bagi, F., Faculty of Agriculture, Novi Sad (Serbia) | Milošević, M., Ministry of Agriculture, Forestry and Water Management of the Republic of Serbia, Belgrade (Serbia) | Vasić, M., Institute of Field and Vegetable Crops, Novi Sad (Serbia) | Vujaković, M., Institute of Field and Vegetable Crops, Novi Sad (Serbia)
Legume production is becoming increasingly important in the Province of Vojvodina (Serbia). The bean varieties grown in Serbia are mostly of domestic origin and must be checked for the presence of harmful pathogens, including viruses. Pathogens causing viral diseases have not been sufficiently studied in the country to date. the present study tested eight bean varieties (Sremac, dvadesetica, Belko, Zlatko, Ribnicanin, Slavonski zeleni, Tisa and Domaca populacija) for infection by the bean common mosaic virus, Bean common mosaic necrosis virus, Bean yellow mosaic virus, Cucumber mosaic virus and Alfalfa mosaic virus at several locations in Vojvodina province. In our study, virus identification in the cultivars was carried out by the DAS ELISA test using a commercial set of antiserums for BCMV, BCMNV, BYMV, CMV and AMV according to the procedure recommended by the manufacturer, LOEWE Biochemica GmbH, Germany. The results of the ELISA test were read on a Multiskan Ascent microplate reader at a wavelength of 405 nm. An extinction coefficient value that was found to be double, that of the negative control was considered a positive result.
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