Can we predict mutagen-induced damage in plant systems mathematically? Insights from zygotic embryo and haploid mutagenesis in Indian mustard (Brassica juncea)
2011
Prem, D., Biological Research Centre (CIB), Madrid (Spain). Plant Development and Nuclear Organization Unit | Gupta, K., Topworth Group of Companies, New Delhi (India) | Agnihotri, A., Amity University Uttar Pradesh, Noida (India). Amity Institute of Microbial Technology
Th is study was undertaken to determine the relationship between mutagen concentration, exposure duration and survival of zygotic embryos along with evaluation of microspore totipotency of the mutant donor plants of Brassica juncea. Three Indian genotypes were tested for varying mutagen concentrations (5-50 mM) and exposure durations (10-50 h) of three chemical mutagens, ethyl methanesulfonate (EMS), ethyl nitrosourea (ENU) and ethidium bromide (EtBr), to study the effect of mutagen exposure on zygotic embryos. The exposure to EtBr resulted in 100% mortality, however, the survival data for EMS/ ENU analyzed by orthogonal contrast partitioning ANOVA revealed that concentration for both mutagens had a linear relationship with percent survival while exposure duration had a non-linear relationship. Multiple regression analysis was used to develop prediction functions for EMS/ ENU treatment survival and the LD50 for zygotic embryo mutagenesis for 20h duration ranged from 3.5mM for ENU to 6.8mM for EMS. Th is information was used to generate mutant donor plants for microspore culture and 48.8% EMS mutant donor plants produced 2.9 +/- 0.4 embryos per Petri dish (total 943 embryos).
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