Fatty acid analysis of Erwinia amylovora from Serbia and Montenegro | Analiza masnih kiselina sojeva Erwinia amylovora iz Srbije i Crne Gore
2011
Ivanović, M., Poljoprivredni fakultet, Beograd - Zemun (Serbia) | Gašić, K., Poljoprivredni fakultet, Beograd - Zemun (Serbia) | Ćalić, A., Poljoprivredni fakultet, Beograd - Zemun (Serbia) | Kuzmanović, N., Poljoprivredni fakultet, Beograd - Zemun (Serbia) | Ivanović, M., Poljoprivredni fakultet, Beograd - Zemun (Serbia) | Obradović, A., Poljoprivredni fakultet, Beograd - Zemun (Serbia)
Automated method of fatty acid analysis was used to identify and study heterogeneity of 41 Erwinia amylovora strains, originating from 8 plant species grown in 13 locations in Serbia and one in Montenegro. All strains contained 14:0 3OH fatty acid, characteristic for the amylovora group. According to fatty acid composition 39 strains were identified as E. amylovora as the first choice from the database. Due to their specific fatty acid composition, two strains were identified as E. amylovora, but as a second choice. Fatty acid analysis also showed that E. amylovora population from Serbia could be differentiated in three groups, designated in this study as alpha, beta and gamma. All strains originating from central or south Serbia, as well as four strains from north Serbia clustered into group alpha. Group beta and gamma contained only strains isolated in northern Serbia (Vojvodina). The results show that E. amylovora population in this area is heterogeneous and indicate pathogen introduction from different directions. Fatty acid analysis enabled identification at species level, as well as new insights of heterogeneity of E. amylovora population.
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