Microbial susceptibility testing method for screening of antibiotics in chicken liver and pork kidney | Ispitivanje osetljivosti mikrobiološke metode za skrining antibiotika u jetri pilića i bubregu svinja
2011
Borović, B., Institut za higijenu i tehnologiju mesa, Beograd (Srbija) | Velebit, B., Institut za higijenu i tehnologiju mesa, Beograd (Srbija) | Đorđević, V., Institut za higijenu i tehnologiju mesa, Beograd (Srbija) | Baltić, T., Institut za higijenu i tehnologiju mesa, Beograd (Srbija) | Spirić, D., Institut za higijenu i tehnologiju mesa, Beograd (Srbija) | Lakićević, B., Institut za higijenu i tehnologiju mesa, Beograd (Srbija) | Janković, V., Institut za higijenu i tehnologiju mesa, Beograd (Srbija)
This paper is part of in-house validation of STAR-protocol for screening of beta-lactam and macrolide residues in liver of broilers and pig kidneys. Detection of antibiotics residues in food requires methods sensitive enough to detect bellow or at MRL value (Maximum Residue Limit). In total 120 blank samples were used from non-treated animals: 60 chickens livers and 60 pig kidneys. Maximum residue limit of antibiotics in tested matrixes are stipulated by Commission Regulation (EU) no. 37/2010, whereas Regulation/Rulebook on quantity of pesticides, metals and metalloids, and other toxic substances, chemotherapeutics, anabolics and other substances found in foodstuffs (Official Journal of SRJ, No. 5/92, 11/92 – corr. and 32/2002), prohibits the antibiotic residues of in meat and meat products. Measuring the width of the growth inhibition zone shows that: in the samples of chicken liver, all of the seven tested beta-lactam antibiotics can be detected at ½ MRL, and higher (zone of inhibition is greater than 4 mm). Erythromycin and tylosin from macrolides group, were detectable at 1/2 MRL, and higher, in the chicken liver matrix (zone of inhibition is greater than 2 mm). The beta-lactam antibiotics in the samples at MRL level and higher can be detected in the kidney of pigs, while the two representatives of macrolides, can be detected in samples at 1/2 MRL.
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