Effect of semen extenders on sperm motion of in vitro stored Muscovy drake spermatozoa
2011
Gerzilov, V., Agricultural University, Plovdiv (Bulgaria). Department of Animal Science | Rashev, P., Bulgarian Academy of Science, Sofia (Bulgaria). Institute of Biology and Immunology of Reproduction | Bochukov, A., Agricultural University, Plovdiv (Bulgaria). Department of Animal Science | Bonchev, P., Agricultural University, Plovdiv (Bulgaria). Department of Animal Science
A study for the influence of semen extenders IMV-buffer, HIA-1 and AU on sperm motion characteristics of Muscovy drake spermatozoa was carried out. The semen from each male (n=6) was divided into three equal parts and diluted in ratio 1:3 (semen:extender) with the IMV-buffer, HIA-1 and AU respectively, and then was stored at temperature 0-4 deg C for 6 hours. Sperm motion parameters - velocity of spermatozoa (rapid, medium, slow and statistic), VCL, VSL, VAP, LIN, STR, WOB were measured using a Sperm Class Analizer (Micropticum, Spain). Computer-assisted sperm motion analysis indicated that Muscovy spermatozoa preserved a rapid and medium sperm velocity after 6 hours in vitro storage in three examination extenders. The total VCL, VSL and VAP of spermatozoa in the semen diluted with AU extender were 110.47 +/- 7.44 microm/s, 29.42 +/- 2.02 microm/s and 57.39 +/- 3.73 microm/s, in the semen diluted with IMV-buffer were 94.93 +/- 11.10 microm/s, 27.57 +/- 2.45 microm/s and 51.35 +/- 4.98 microm/s, and in the semen diluted with HIA-1 were 68.48 +/- 12.74 microm/s, 20.08 +/- 4.18 microm/s and 37.75 +/- 7.65 microm/s, as the differences were significant between AU and HIA-1 - (P is less than 0.05). About LIN, STR, WOB there were no significant differences for the influence of the extenders.
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