Use of enterocin S760 for prevention and treatment of experimental salmonella infection in mice | Использование энтероцина S760 для профилактики и лечения экспериментальной сальмонеллезной инфекции у мышей
2010
Svetoch, Eh.A. | Borzilov, A.I. | Eruslanov, B.V. | Korobova, O.V. | Tejmurazov, M.G. | Kombarova, T.I. | Dyatlov, I.A., State Research Center of Applied Microbiology and Biotechnology, Moscow Region (Russian Federation)
Shown is the treatment efficacy of bacteriocin S760 synthesized by Enterococcus faecium 760 for septic Salmonella infection in mice. One hundred mice, which were intraperitoneally inoculated with 100 LD50 of Salmonella enteritidis strain 92 Rif, received bacteriocin 1 hour (prevention) or 48 hours (treatment) after inoculation in doses 25, 50, or 100 mg/kg every 6 hours during 5 or 10 days. The efficiency of was evaluated by the number of survived mice and by the degree of contamination of their organs and blood with S. enteritidis strain 92 Rif 14 days after the treatment termination. The high degree of bacterial contamination of liver, spleen and blood was observed already 1 h after inoculation and predetermined the proposed bacteriocin treatment scheme. Parenteral administration of bacteriocin 1 h after inoculation at the dose of 50 mg/kg every 6 h for 10 days showed 100% survivability of mice, wherein parenchyma organs and blood of animals 4 days after the treatment were free of S. enteritidis strain 92 Rif. Use of peptide S760 for prophylaxis in dose 50 mg/kg during 10 days prevented lethal infection in 100% of animals, whereas its use for treatment cured 70% of animals with generalized salmonellosis. Shortening of treatment course from 10 to 5 days and reducing dose of bacteriocin led to less pronounced treatment effect but in all animals it was expressed by increase of mean length of life compared to control (not treated). Obtained results demonstrated high treatment efficacy of bacteriocin S760 during septic salmonellosis and perspectives of its use in medicine and animal health
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