Influence of nutrition sources on entomopathogenic fungus Lecanicillium lecanii Zare and W. Gams productivity | Влияние источников питания на продуктивность энтомопатогенного гриба Lecanicillium lecanii Zare and W. Gams
2011
Prishchepa, L.I. | Vojtka, D.V. | Ugnacheva, E.V., National Academy of Sciences. Scientific and Practical Center for Arable Farming (Belarus). Institute of Plant Protection
In course of the research there was studied the nutrition requirement of a fungus Lecanicillium lecanii Zare and W. Gams (Verticillium lecanii) in the basic nutrition supplies. The research was realized in the conditions of the Republic of Belarus in 2007-2009. In the works the strains L. lecanii (BL-1, BL-2, Vc-1, ПV-1) from the collections of the Institute of Plant Protection of the National Academy of Sciences of Belarus were used. The analysis of experimental results has shown that L. lecanii strains differentially used carbon sources. So, sorbite, starch and glycerine provided the maximum sporogenesis for all strains. For L. lecanii strains inorganic sources of nitrogen were preferable: ammonium and nitrate nitrogen. As a result of realized research it was determined that the maximum spore titer was obtained on the third-fourth day of submerged cultivation. On the obtained data basis the balanced medium for industrial cultivation, containing melasses, glycerine, hydrolysate derty and mineral salts was offered. Efficiency of L. lecanii BL-2 strain on the optimum medium has made 2.5x10 spores/ml on the 4-th day of cultivation.
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