Expression of MMP-2, MMP-9,TIMP-1 and TIMP-2 in tanapox virus infecting OMK cells and their relationships with tanapox virus infection | MMP-2、MMP-9及TIMP-1、TIMP-2在TPV感染OMK细胞中的表达及其与感染的关系

中国人. 研究在特纳河痘病毒(Tanapox virus,TPV)感染枭猴肾上皮细胞(Owl monkey kidney epithelial cells,OMK)过程中，基质金属蛋白酶-2 (Matrix metalloproteinase-2,MMP-2)、MMP-9和其组织抑制剂(Tissue inhibitor of metalloproteinase，TIMP）TIMP-1、TIMP-2的表达变化及其与感染的关系。用MMPs高特异性抑制剂（MMP-2Ⅱ、MMP-9Ⅰ）降低MMP-2或MMP-9的活性，研究其对TPV感染的影响和对OMK细胞的毒性。采用明胶酶谱法检测MMP-9和MMP-2在TPV感染过程中蛋白质水平的变化，并应用实时荧光定量PCR方法检测TPV感染中TIMP-1和TIMP-2 mRNA 表达水平的变化。MMP-2和MMP-9的高特异性抑制剂对TPV感染具有显著抑制效果，且对OMK细胞没有毒性作用。在TPV感染OMK细胞过程中，MMP-2和MMP-9蛋白水平升高，而TIMP-1和TIMP-2的mRNA表达水平下降。MMP-2和MMP-9参与TPV感染，可能对病毒的感染具有促进作用。MMP-2、MMP-9和TIMP-1、TIMP-2比例失衡，造成MMP-2和MMP-9相对“过盛”，这可能是MMPs促进TPV感染的重要机制。

英语. The study was to examine the expression of matrix metalloproteinase-2, 9 (MMP-2, 9) and tissue inhibitors of metalloproteinase-1, 2 (TIMP-1, 2) in Tanapox virus (TPV) infection and their relationship with TPV infection. The inhibitory effects of MMP-2 or MMP-9 high selective inhibitors on TPV infection and the cell toxicity of them in OMK cell were tested respectively. Gelatin zymogaphy and Real time PCR were applied to evaluate the protein levels of MMP-2, 9 and the mRNA levels of TIMP-1, 2, respectively. TPV infection was inhibited by MMP-2 and MMP-9 inhibitors through decreasing the MMPs activity without OMK cell toxicity. The protein levels of MMP-2 and MMP-9 were up regulated during TPV infection. At the same time, the mRNA levels of TIMP-1 and TIMP-2 were down regulated during TPV infection. MMP-2 and MMP-9 play an important role in TPV infection and possible are factors to promote the infection. Perhaps TPV infection is associated with imbalance of MMPs/TIMPs, especially MMP-9, MMP-2, TIMP-1 and TIMP-2.