Assessment of ranges plasma indices in rainbow trout (Oncorhynchus mykiss) reared under conditions of intensive aquaculture
2011
Kopp, R., Mendelova Univ., Brno (Czech Republic). Ustav Zoologie, Rybarstvi, Hydrobiologie a Vcelarstvi | Mares, J., Mendelova Univ., Brno (Czech Republic). Ustav Zoologie, Rybarstvi, Hydrobiologie a Vcelarstvi | Lang, S., Mendelova Univ., Brno (Czech Republic). Ustav Zoologie, Rybarstvi, Hydrobiologie a Vcelarstvi | Brabec, T., Mendelova Univ., Brno (Czech Republic). Ustav Zoologie, Rybarstvi, Hydrobiologie a Vcelarstvi | Zikova, A., Leibniz-Inst. of Freshwater Ecology and Inland Fisheries, Berlin (Germany). Dept. of Physiology and Aquaculture
Plasma composition in Oncorhynchus mykiss from three various trout farms in the Czech Republic was assessed using automated blood plasma analyser. Non-haemolysed serum from the heart of 48 healthy, randomly selected fish (standard length: 247.3+/-24.2 mm; body mass: 262.18+/-87.28 g) was analysed for the following variables: alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase, acid phosphatase, lactate dehydrogenase, creatine kinase, total protein, cholinesterase, amylase, glucose, lactate, albumin, urea, cholesterol, triglycerides, lipase, Ca, Mg, P, Fe, Na, K and Cl. All data were analysed statistically. With regard to data distribution, 19 indices out of 23 (aspartate aminotransferase, alkaline phosphatase, acid phosphatase, lactate dehydrogenase, total protein, amylase, glucose, lactate, albumin, urea, cholesterol, triglycerides, Ca, Mg, P, Fe, Na, K and Cl) were normally distributed. The indices were affected by handling time and, accordingly, to the physical and chemical properties of water. Estimates obtained were compared with previously reported ranges. The blood automated analyser proved to be a valuable and reliable instrument for the estimation of plasma composition determining normal ranges in rainbow trout.
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