Relationship of ejaculate quality and selected biochemical parameters of blood in cockerels of three laying lines
2012
Jarinkovicova, L., Mendelova Univ., Brno (Czech Republic). Agronomicka Fakulta | Machal, L., Mendelova Univ., Brno (Czech Republic). Agronomicka Fakulta | Machal, J., Masarykova Univ., Brno (Czech Republic). Ustav Patologicke Fyziologie | Filipcik, R., Mendelova Univ., Brno (Czech Republic). Agronomicka Fakulta | Tumova, E., Ceska Zemedelska Univ., Prague (Czech Republic). Fakulta Agrobiologie, Potravinovych a Prirodnich Zdroju | Horsky, R., Mendelova Univ., Brno (Czech Republic). Agronomicka Fakulta
Ejaculates and blood plasma were sampled from cocks of three laying lines: Barred Plymouth Rock (BPR), Sussex Light (SU), and Rhode Island Red (RIR). Ejaculates and blood plasma were sampled four times during the laying period of hens. The following ejaculate characteristics were determined: sperm motility, concentrations of sperm cells, ejaculate volume. Sperm morphology was examined. In the blood samples, concentrations of glucose, cholesterol, creatinine, calcium, phosphorus, and magnesium were analysed. The motility of spermatozoa of the cocks was 47.0% (BPR), 47.7% (RIR), and 48.3% (SU), respectively. The highest volume of ejaculate was found in BPR line (0.66 cubic cm), the lowest one in SU line (0.46 cubic cm, P less than 0.01); the highest sperm concentration was in SU line (2.46 x 10**6/cubic mm), the lowest one in RIR line (1.96 x 10**6/cubic mm, P less than 0.01). The number of morphologically abnormal sperm cells was similar in all lines - 47.0% BPR, 47.7% RIR, and 48.3% SU, respectively. In general, the occurrence of defective spermatozoa was high in all three lines; the most frequent were tail defects (from 20.3 to 29.7%), while sperm cells with developmental anomalies were less frequent (only 0.3 to 0.4%). Calculated phenotypic correlation between sperm motility on the one hand and the occurrence of defective sperm cells on the other was negative (rp = -0.28, P less than 0.01), as well as the correlation between sperm motility and sperm numbers, and between sperm motility and ejaculate volume (rp = -0.28, P less than 0.01 and rp = -0.31, P less than 0.01, respectively). Negative correlations were found between the level of Mg in blood plasma and numbers of morphologically defective spermatozoa in the ejaculate, defective heads and defective connecting pieces (rp = -0.33, P less than 0.01; rp = -0.23, P less than 0.05; and rp = -0.26, P less than 0.05). Mg level was positively correlated to sperm motility (rp = 0.26, P less than 0.05). However, positive correlations existed between concentration of glucose in blood plasma of cocks and numbers of morphologically defective spermatozoa in sampled ejaculates (rp = 0.27, P less than 0.01).
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