Assessment of faba bean rhizobium diversity in Sharkia Governorate areas based on symbiotic traits of biological nitrogen-fixation
2009
Abdel Basit, H.M.L.
In order to contribute for the optimization of biological nitrogen fixation (BNF) associated with faba bean plants, this study aimed to analyze the diversity of rhizobial populations (Rhizobium leguminosarum bv. viciae) in eight areas of the Sharkia governorate. Symbiotic traits of biological nitrogen-fixation were determined for a collection of rhizobia captured using faba bean as a host plant. A computer numerical analysis of data was carried out. An assessment of diversity among rhizobial isolates based on symbiotic traits of biological nitrogen fixation (no. of nodules/plant, dry weight of nodules, nitrogenase activity, dry weight of roots and shoots as well as their total nitrogen content) showed that the highest distance value was found between isolates RZ11 and RK11. The minimum genetic distance was found between RR13 and RA21 also between RB2 and RB3 isolate. The data indicated that isolates RZ11 and RK11 were the most distant isolates. The obtained dendrogram discriminates all the Rhizobium leguminosarum bv. viciae populations examined. Diversity analysis of the studied 27 Rhizobium populations showed high levels of genetic diversity among these populations and sub-divided them into nine main groups with subdivision into sub-groups and confirmed that the isolate RZ11 was the most divergent. The data indicated that symbiotic traits of biological nitrogen fixation can provide a relatively unbiased method of quantifying genetic diversity among isolates of indigenous populations of rhizobia nodulating faba bean in Sharkia soils. This study provides information on the diversity of indigenous populationsof rhizobia (Rhizobium Leguminosarum bv. Viciae), which has practical implication for applying biological nitrogen fixation in plant production.
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