Ovarian morphology and oocyte quality in relation to in vitro embryo production in ruminant animals
2009
Mohammed, A.N .A.
The aim of this study was to evaluate the reproductive status of small (sheep and goat) and large ruminants (cow and buffalo) slaughtered out of slaughterhouse through the characteristics of ovaries and oocytes. Ages of slaughtered animals were determined through the teeth. They were classified into young with milk teeth and adult with 2-8 permanent incisors. Genitalia were taken immediately after slaughtering the animals. The collected genitalia were normal and from non-pregnant animals, Ovaries were examined to determine the reproductive status according to the estrus cycle. Ovaries were classified into follicular or luteal phase. Weight of reproductive systems and ovaries as well as the number of follicles per animals were recorded. Oocyte were aspirated from the large follicles using an 18-gauge needle and examined under a stereomicroscope to identify and evaluate the quality of oocytes morphologically. Weights of ovaries were increased with increasing weights of reproductive systems.Also,weights of ovaries were increased significantly (P0.05)during the luteal phase compared to the follicular phase. Most of the examined ovaries were showed large number offollicles. The results indicated that the majority of the collected oocytes were evaluated and classified morphologically as grade I. The collected oocytes can be used for in vitro production of embryos and other purposes
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