Use of RAPD markers for characterization of rice (Oryza sativa L.) varieties
2009
Bhartiya, Anuradha | Mani, S.C. | Johri, Tanu | Pande, Kamal K.
Characterization of nineteen rice varieties was undertaken using twelve RAPD markers. A total of 68 amplicons were obtained with an average of 5.6 bands per primer. Of these, 48 were found to be polymorphic and the level of polymorphism was 70.58. The number of bands generated were more primer dependent than on the genotype and ranged from 1 to 11. The per centage of polymorphic bands ranged from 50% (EO 1598, EO 1594) to 100 % (EO 1600, EO 1596). Though all the genotypes were discriminated in the present study still the identification of genotypes was not possible with a limited number of primers. A few of the primers generated very specific bands for only a limited number of genotypes. Jaccard's pair wise similarity coefficients of values for 19 genotypes were calculated and the range of genetic similarity was found to be 0.65 to 0.92 with an average of 0.75 + 0.05. A dendrogram was generated by UPGMA cluster analysis based on Jaccard's similarity coefficients which showed poor grouping of genotypes because of the similarity among the varieties.
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