Application of isozyme markers for DUS testing for rice (Oryza sativa L.) varieties
2009
Bhartiya, Anuradha | Mani, S. C. | Johri, Tanu | Pande, Kamal K.
Plant morphological characters have been the universally undisputed descriptor for genotype characterization. But these descriptors, besides being limited in number, make the process time consuming and also less reliable, owing to their interaction with the environment in which the variety is grown. In this regard the potential of biochemical descriptor like seed esterase, peroxidases, malate dehydrogenases and alcohol dehydrogenases, merits investigation. Isozyme has been proven to be more powerful as genetic marker for studies of plant population than morphological characters. More information can be obtained from isozyme marker than morphological markers since more loci are detected and isozymes are codominant markers. Genetic inheritance of isozyme is also easier to determine and allelic frequency can be calculated directly. Isozymes have also been found to be independent of environmental influences.
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