Clonal propagation of selected sugar beet breeding lines in in vitro culture
2010
Norouzi, P. | Sadeghian, S.Y. | Mahmoudi, S.B. | Banisadr, F. | Bigdeli, M. | Koohestani, M.
It is important to keep characteristics of parental lines of hybrid variety. Increasment of these lines by seed production resulted in changes of genotype and combineability. Hence, micropropagation technique can almost overcome these problems. Therefore, it can be obtained stock seed adequetly with retained characteristics for producing elite seed and then pollinator paprent of hybrid variety by clonal propagation. In this project, the selected breeding lines (pollinator 191 and line S1-63 from SB19 that had superiror hybrids in yield trial in rhizoctonia infected condition). For this, in first year, about 5 gr the seed 191 surface sterilized and cultured in in vitro culture. 50 -100 shoot apices excised from 15 dayes-old seedlings and cultured onto medium to propagate the tissue culture clones. In the next step, the clones were transferred to rooting medium and then they transferred to small pots in laboratory and transferred to bigger pots in greenhouse for adaptation. In winter, vernalization of the clones was done at cold room at Sugar Beet Seed Institute (SBSI) in Karaj. In second year, the vernalized clones were transferred to isolate plots in the field and harvested polycross seeds from the clones of line-191. In this year, also clonal propagation of S1-63 line was done and adapted clones to greenhouse or field conditions vernalized at cold room in winter. In tirth year, vernalized plants from each independent clone number were separately transferred to isolate plots in Karaj. The seed was sufficiently prepared from some independent clones of S1-63 breeding line. Therefore, in this project, the seed obtained from tissue culture-clones for pollinator-191 and breeding line S1-63 was prepared at sugar beet seed institute as a first report and delivered to breeding department of SBSI.
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