Determination of paraquat residue in soil by high performance liquid chromatography
2008
Wahyu Wibawa | Rosli Mohamad, Putra Malaysia Univ. (UPM), 43400 UPM Serdang, Selangor (Malaysia). Faculty of Agriculture. Dept. of Crop Science | Dzolkhifli Omar, Putra Malaysia Univ. (UPM), 43400 UPM Serdang, Selangor (Malaysia). Faculty of Agriculture. Dept. of Plant Protection | Adam Puteh | Mohammad Mohd. Lassim, Putra Malaysia Univ. (UPM), 43400 UPM Serdang, Selangor (Malaysia). Faculty of Agriculture. Dept. of Crop Science
A high performance liquid chromatography (HPLC) method with short and narrow C18 column (3.9 x 150 mm i.d.) was used to determine paraquat (1,1'-dimethyl-4,4'-dipyridinium chloride) residue content in soil. Standard stock solution was used for calibration curve determination and spiking the soil. Residues were extracted and clean-up from the spiked soil for analysis by HPLC to determine the separation capability and efficiency of the analytical procedure. The response of paraquat working standard concentration to the recovery in the calibration curve was in reasonable agreement with the linear regression equation of y = 97244x - 6.8697, and R2 = 0.9989. The detection limit obtained was at 0.5 ppm, but the retention time was short at 1.31 min. of the 5 min. running time. These performances showed that the extraction procedure and HPLC system were able to separate paraquat at reasonable limit, and could provide an alternative technique for determ ining paraquat residue concentration in soil.
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