Effect of ..-Mercaptoethanol on in vitro Maturation of Porcine Follicular Oocytes and Development of Porcine IVM/IVF Embryos.
2003
Han M.H. | Lee K.B. | Cheon H.S. | Park B.K. | Seo K.W. | Lee K.S.
The present study was carried out to examine the effect of ..-mercaptoethanol on in vitro maturation of porcine follicular oocytes and oxygen concentration with ..-ME on vitro development of porcine IVM/IVF embryos. The rates of nuclear maturation, penetrated oocytes , polyspermic oocytes, pronucleus formation and mean numbers of the penetrated sperms were not significantly different using NCSU-23 maturation media for 0,25, 50 and 100.. ..-ME. The rates of blastocyst formation at day 7 after in vitro fertilization were higher in oocytes matured with 25 .. ..-ME than in those matured with 0, 50, and 100.. .However, no differences ware found in total cell numbers of blastocyst among the retreatments. The rates of blastocyst formation at day 7 after in vitro fertilization were higher than in the NCSU-23 culture medium with 25.. ..-ME than in those cultured with 0, 12.5, and 50.. ..-ME under the 5% and 20% O.. concentrations.
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