Le brunissement du bois de la vigne : mise au point des methodes de diagnostic mol.culaires pour Phaeomoniella chlamydospora.
2004
Bensadok D. F.
Wood browning of grapevine is one of the symptoms associated to esca disease on the young plants and propagating material. Phaeomoniella chlamydospora is one of the fungi usually isolated from brown wood. A DNA extraction protocol was set up for Phaeomoniella chlamydospora molecular detection in the plant tissues and in the soil that allowed, through Nested PCR, to detect the pathogen at a concentration of 1 pg DNA for plant material and of 1.6 x 10sup2 conidia/gram in the soil. The application of this technique to grafted and ungrafted rootstocks (776 plants) taken in different nurseries (Algeria and ltaly) enabled to detect the presence of Phaeomoniella chlamydospora with a frequency of 40 and 20% respectively. The pathogen was also detected in the soil samples. These techniques are not knowledge-demanding in terms of fungal taxonomy; they are fast and applicable to a large number of samples. It is therefore recommended to apply them for a large-scale monitoring with a view to assessing the health status of the propagating material.
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