Studies on in vitro maturation of bovine follicular oocytes, 1; collection and in vitro culture of follicular oocytes.
1989
Yoon S.H. | Ko D.H. | Park S.P. | Park T.G. | Chung K.S.
These experiments were carried out to develop the effective recovering method of ovarian follicular oocytes and to investigate the optimum culture conditions for in vitro maturation of recovered follicular oocytes. Bovine follicular oocytes with various configurations of cumulus cell mass were collected by aspirating follicular fluid from the ovarian follicles of various sizes, and then cultured in various media for 28h in an incubator with 5 % CO2 in air at 38deg C. The average number of the follicular oocytes recovered from a cow was 46.4 and the highest recovery rate was obtained from middle size follicles, 3-5mm in diameter. The majority of the follicular oocytes with compacted cumulus cells existed in GV stage while those with dispersed cumulus cells or without cumulus cells existed between GVBD and MII stage. The extrusion rate of 1st polar body of the follicular oocytes cultured in Ham's F-10, m-KRB and BMOC-3 containing hormones was not higher than those of oocytes cultured in those media without hormones. However, in case of m-TALP medium the highest extrusion rate of 1st polar body, 77.7 % was obtained by adding hormones such as FSH, HCG and Estradiol. The maturation rate of the follicular oocytes cultured in basic medium without or with matured cumulus cell was 40 % or 51.1 %, respectively, with significant difference (P<0.05).
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