Methods used to screen maize for and to determine mechanisms of resistance to the Southwestern corn borer and fall armyworm.
1989
Davis F.M. | Williams W.P. | Wiseman B.R.
This paper describes methods used to screen maize for resistance to the southwestern corn borer, Diatraea grandiosella Dyar, and the fall armyworm, Spodoptera frugiperda (J.E. Smith) and to determine the mechanism(s) responsible for resistance. Maize genotypes are screened in the field for leaf feeding resistance to both insects by infesting plants, using the "Bazooka" technique, in the mid-whorl stage of development with laboratory reared larvae. The plants are evaluated for damage using a visual leaf-feeding damage rating 14 days after infestation. The mechanisms of resistance are identified by conducting choice and no-choice feeding experiments in the laboratory. The techniques involve the use of excised leaf tissue or tissue incorporated into an artificial diet as a food source. The use of callus derived from maize genotypes is also discussed for small-scale screening to lepidopterous pests and for determining mechanisms and bases of resistance.
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