Genotypic differences in salt tolerance of tissue cultures in Tomato species.
1989
Liu Kebin | Li Shuxuan
Tissue cultures of 24 cultivars and 2 varieties of Lycopersicon esculentum and wild tomato species L. pimpinellifolium were used as materials and cultured in vitro for the studies on salt tolerance and genotypic responses to salinity. The present results available on salt tolerance seemed to indicate that mechanisms of salt tolerance of tomato leaf cultures and callus were concerned with: 1. osmoregulation by mineral ions Na+, Cl- and organic solute proline, 2. growth retardation and adaptability increase distinguished by decrease in total soluble protein level, appearance of specific proteins and peroxidase isozymes and increased peroxidase isozyme activity. It was noted that there existed significant genotypic differences in salt tolerance among the 27 genotypes tested, and the relative salt-tolerant genotypes were characterized by the high Na+, Cl- and proline accumulation and high succulence in their leaf cultures and calli.
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