Species-specific detection of Listeria monocytogenes by DNA amplification.
1991
Deneer H.G. | Boychuk I.
The polymerase chain reaction was used to detect and specifically identify Listeria monocytogenes. A 174-bp region of the listeriolysin O gene was shown to be specifically amplified in L. monocytogenes but not in other species of Listeria or in a number of other gram-positive and gram-negative organisms. Less than 50 organisms could routinely be detected by a procedure involving two rounds of 35 amplification cycles each and without the need for subsequent hybridization with labeled probes.
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书目信息
Applied and environmental microbiology
卷
57
ISSN
0099-2240
页码
v.606-609(2)
其它主题
Diagnostico; Bioquimica
语言
英语
注释
ill. references. AVAILABILITY: US (DNAL 448.3 AP5).
类型
Journal Article; Journal Part
来源
Applied-and-environmental-microbiology (USA). (Feb 1991). v. 57(2) p. 606-609.
团体作者
University of Saskatchewan, Saskatoon, Saskatchewan, Canada
2012-11-15
AGRIS AP