Growth pattern and enterotoxin production of food poisoning bacteria in fish-fillets stored under different modified atmospheres.
1992
Oka S. | Ito H. | Takama K.
Bigeye tuna (Thunnus obesus) fillets and toxigenic food poisoning bacterium (Escherichia coli, Staphylococcus aureus and Clostridium perfringens, specifically) were packed into retort pouches and separately filled with Air and Gas mixture (CO2/N2 60:40), respectively. The growth pattern and enterotoxin production of these organisms in both packed samples were examined during storage for 5 days at 25 degrees centigrade. After 5 days, the numbers of E. coli and S. aureus in Air packed samples were 1.2x10(9)/g and 1.4x10(7)/g flesh, and 1.6x10(8)/g and 1.6x10(5)/g flesh in Gas packed samples, respectively. The results showed that growth of these organisms were slightly inhibited with the Gas mixture. However, the number of C. perfringens was 1.7x10(7)/g and 3.7x10(7)/g flesh in both the Air and Gas packed samples, respectively. No inhibition was observed for the growth of this organisms under Gas packed condition. Each of the 9 strains of E. coli and S. aureus from both packed samples produced the toxins of 5ng/ml and 1,000ng/ml culture supernatant. The Gas packaging did not show any inhibitions for the production of their toxins. All of tested strains of C. perfringens produced the toxin of 10,000ng/ml culture supernatant and the toxin production was not controlled in the Gas package, too. From these results, it was concluded that Gas packaging slightly inhibited the growth of the tested toxigenic food poisoning bacteria except C. perfringens, but not for their toxin productions.
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