Selection of salt-tolerant callus from eggplant [Solanum melongena] hypocotyl.
1992
Murakami K. | Matsubara S.
Hypocotyl segments of eggplant were cultured on media supplied with various concentrations of NaCl. The fresh weight of callus on 0.2 % NaCl medium dropped to half of that found on a NaCl-free medium. Callus was not initiated on media containing more than 1.0 % NaCl. Callus on 0.8 % NaCl was chosen for further selection. After subculturing on a 0.8 % NaCl medium for 2 years, tolerant callus grew vigorously and named C-0.8. C-0.8 callus then sequentially transferred to media containing increasing levels of NaCl (0.8, 1.0, 1.2, 1.4 and 1.6 % NaCl). Surviving callus was named C-1.6. Control callus was obtained by culturing on a Nacl-free medium for 2 years (C-0). The color of C-0.8 and C-1.6 was green, whereas that of C-0 was white. C-0.8 was more friable than C-0, whereas C-1.6 was more compact than C-0. The dry matter content of C-1.6 was higher than that of C-0.8 and C-0. On the 0.8 % NaCl medium, the growth of C-0 was strongly inhibited but that of C-0.8 and C-1.6 was only slightly inhibited. The stability of the salt tolerance was examined by transferring calli to a NaCl-free media for 60 days. Treated C-0.8 retained the trait of salt tolerance, but c-1.6 callus lost its salt tolerance. Adventitious shoots regenerated from C-0.8 after their transfer to a NaCl-free medium containing 1.0 mg/liter BA. Thus the salt tolerant callus of eggplant was obtained by its subculture on a medium containing 0.8 % NaCl.
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