CryIA(b) gene from Bacillus thuringiensis subsp. wuhanensis, DNA sequence analysis and insecticidal activity.
1994
Nakatani I. | Asano S. | Bando H. | Iizuka T.
Nucleotide sequence of cryIA(b) gene from B. thuringiensis subsp. wuhanensis (cryIA(b)-W) was determined and compared with other two cryIA(b) genes, one from subsp. kurstaki HD-1 (cryIA(b)-H) and other from subsp. thuringiensis berliner 1715 (cryIA(b)-T). The deduced 564 amino acid sequence of cryIA(b)-W was extremely similar to that of cryIA(b)-T, with an exception of amino acid number 207. The entire coding sequence of cryIA(b)-W and cryIA(b)-H were cloned separately into pKK233-2 and insecticidal proteins (ICP) were expressed in Escherichia coli JM109. After sonication of E. coli cells, solubilized ICPs were directly injected to the mouth of 5th-instar larvae of the silkworm, Bombyx mori. The bioassay results indicated that the ICPs had no toxicity against the silkworm. Iizuka et al. (1992) reported that subsp. wuhanensis contained additional two cryI genes, cryIA(c) and cryID. It should be of interest to determine the nucleotide sequence of these two genes and the insecticidal activity of the gene products.
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