Uptake of yolk protein by housefly ovaries in vitro culture.
1994
Gong He | Zheng Wenhui
Experiments were performed to study the incorporation of yolk protein labelled with fluorescein isothiocyanate (FITC) into housefly follicles in vitro. It was revealed that the rate of yolk protein incorporation depended on the concentration of yolk protein in the culture medium and temperature. The optimal condition for yolk protein incorporation in this Grace's medium was 2 mg yolk protein per ml culture medium at 27 deg C for two hours. The capacity of follicles in yolk protein uptake reached summit when housefly ovaries developed to the stage 6-8 about 48 hours after adult emergence. Juvenile hormone (JH III), if added to the culture medium at concentration of 5.9--117.3 ng/mul, could increase the amount of yolk protein incorporated into ovarian follicles. The yolk protein uptake by follicles in vitro was inhibited by ouabain, bovine serum albumin (BSA) or sodium azide.
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