Continuous exposure of cells to a hormonal stimulus results in attenuation of the hormone's effects on the cell; a process known as desensitization. The present study was undertaken to determine whether glucagon (GLU) induces desensitization of its lipolytic effect in adipocytes isolated from the abdominal fat of market-age broilers. Preincubation of adipocytes with 10 to 100 ng/mL of porcine GLU (pGLU) or chicken GLU (cGLU) for 24 h reduced (P < 0.05) GLU-stimulated lipolysis. However, pGLU decreased (P < 0.05) lipolysis to a greater extent than cGLU. Maximal lipolysis was reduced 70% by pGLU and 55% by cGLU. Chicken GLU also exhibited lower biological potency for acutely stimulating lipolysis from control and cGLU-treated adipocytes. Glycerol release from control adipocytes incubated for 1 h with 0.3 ng/mL of cGLU or pGLU was 26 and 42 nmol/h per 3% cells, respectively. The GLU-induced decrease in lipolysis occurred rapidly and was partially reversible. The results of the present study indicated that GLU induced desensitization of its lipolytic effect in broiler adipocytes.
显示更多 [+] 显示较少 [-]
AGRIS - 国际农业科技情报系统
Share
页码
v.1015-1021(6)
其它主题
Adipose tissues; Cultivo de celulas; Lipolisis; Celulas
语言
英语
注释
references. AVAILABILITY: US (DNAL 47.8 AM33P).
类型
Journal Article; Journal Part
来源
Poultry-science (USA). (Jun 1992). v. 71(6) p. 1015-1021.
团体作者
USDA, ARS, Poultry Research Laboratory, Georgetown, DE
2012-11-15
AGRIS AP