Study on the establishment of disease-free trees and nursery trees of citrus in China.
1987
Ke Chung | Lin Xan Zhan
Parent source trees were selected from various citrus areas in Fujian [China]. Budsticks taken from each parent source trees were treated by hot-moist air, antibiotic and tip-stem micrograft for an attempt to eradicate Hunglungbin pathogen and other bud carried viruses. The treated buds were grafted on to rootstocks grown from seeds previously disinfected by hot water treatment for propagating mother tree candidates. The mother tree were indexed using three methods; i.e., electron microscope examination, biological and serological tests for detection of virus and virus-like pathogen such as Hunglungbin prokaryote, tristiza virus and exocortis viroid which are present in China. Buds were taken from these disease-free mother trees in the succeeding years for propagating disease-free nursery trees by grafting them in rootstocks grown from hot water treated seeds. At present, 5,000 disease-free mother trees from 11 parent source trees of sweet orange, Pankan, Tankan and Mandarin citrus cultivars were planted in the mother tree foundation, and about 20,000 nursery trees were propagated and planted in various areas. These are all free from the above-mentioned disease.
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