Comparacion de dos tecnicas para coleccion y tincion de muestras vaginales en la gata.

The present study was realized between September and December of 1992 in the Veterinary Medicine Faculty's laboratories of the Concepcion University, Chillan, Chile. The objective of this work was to compare two distinct stains and collection's methods of vaginal smears in the queen, by using cotton swabs or hyssop and dropper for vaginal irrigation. The Papanicolaou's and Giemsa's methods also were compared, and the efficiency between stains was evaluated being the first considered as a pattern. The required animals, twenty-four, were obtained in Small Animals Clinic and its particulars address. The queens were divided into 2 sub-groups in order that each sub-group received one of the two methods of vaginal sampling. To compare the collection technics, three factors were evaluated: -Quality and facility to obtain samples. -Number of cells obtained using each method (requiring a minimum of 100 cells). -Alternated cells percentage obtained in each method, in the Giemsa stained smears. Thus, by each collection method, two smears were obtained and each one of them was subject to different stain (Papanicolaou and Giemsa), in order to compare each stain method through determination of mature index (M.I.) and ratio of intermediate cells present in each vaginal smear. In collection techniques analysis, was concluded the use of cotton swabs in the collection of queen's vaginal smears, represents a simple, easy, and fast method, in respect, to use of vaginal irrigation. The number of cells obtained with both methods was enough to determine the mature index, although the cells were presented more isolately. The number of alternated cells (cytolysis and folding) is less using vaginal irrigation. However, the statistical analysis proved the absence of significative differences in the total alternated cells obtained by one or another method. By comparing both stain techniques, first, it was determined the mature index through, the revision and classification of the parabasal, intermediate and superficial cells to check 100 of them in each vaginal smear.