Leucosis enzootica bovina (LEB): Estudio comparativo de diagnostico por el metodo de inmunodifision en gel agar (IGA) y el ensayo de inmunoabsorcion enzimatica (ELISA).
1992
Aguilar M Fernando Javier
Enzootic Bovine Leukosis is a neoplasia affecting leukocytes forming tissue and its etiologic agent is BLV (Bovine Leukemia Virus). The diagnosis of the disease is mainly based on the detection of the anti-BLV antibodies in sera of infected animals, through serological tests. The present study compares the diagnosis sensitivity between the Agar Gel Immuno Diffusion Test (AGID-Test) and the Enzyme-Linked Immuno Sorbant Assay (ELISA). With this objective, sera were taken from cows coming from herds with high incidence of the disease, in a dairy district of the province of Rancagua. Among the 110 sera tested with both tests, there was a total agreement of results in 100 (91%) samples, and disagreement ones 10 samples (9%) which were positive to ELISA and negative to AGID. The Sensitivity of the AGID, compared with the ELISA, was 85.3% and the Specificity was 100%. Reading the agar-gel plates at 72 hours clarified those reactions that were weakly positive at 48 hours. The high sensitivity showed by the ELISA would allow more reliable results in Control and Eradication Programme of the Disease in our country.
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