Tagging and mapping the thermosensitive genic male sterile gene of rice with molecular markers.
1996
Wang B. | Xu W.W. | Wang J.Z. | Weng M.L. | Li M.Y. | Yang R.C. | Nguyen H.T.
Bulk segregant analysis of F2 populations was used to identify random amplified polymorphic DNA (RAPD) markers linked to the thermosensitive genic male sterile (TGMS) gene of rice. Four hundred RAPD primers were screened for polymorphisms between the parents and between the two bulk populations representing fertile and sterile plants. Of these, four primers produced polymorphic products. Most of the polymorphic fragments contained repetitive sequences. Only one single-copy sequence fragment was found, a 1.2-kb fragment amplified by primer OPB-19, and subsequently named TGMS1.2. TGMS1.2 was mapped on chromosome 8 with a genetic distance of about 6.7 cM from the TGMS gene. TGMS1.2 was sequenced and changed to a sequence-tagged site marker. It is available for public use. A rice bacterial artificial chromosome library was screened with the labeled TGMS 1.2 as the probe. Nine positive clones were screened.
显示更多 [+] 显示较少 [-]