QTL analysis of yield traits in a recombinant inbred population
2011
Niño, M.C. | Tabanao, D.A. | Rico, E.P. Jr. | Duque, M.J.C., Philippine Rice Research Inst., Maligaya, Science City of Muñoz, 3119 Nueva Ecija (Philippines)
As a complex agronomic trait, grain yield is a result of the combined effects of many genes, each having a small effect that is sensitive to environmental changes. This study aimed to identify quantitative trait loci (QTL) associated with yield traits in a recombinant inbred population. Recombinant inbred lines derived from an F2 population were developed for QTL mapping. The population was laid out in an augmented block design with nine sets in 2009 DS and 2010 DS. A total of 105 simple sequence repeats (SSR) markers, spaced at 3 Mb, were used for genotyping. Eight putative QTL above a threshold LOD score of 2.5 were detected by composite interval mapping (CIM) in each season. High-yield associated alleles were detected from both parents. The number of QTL detected were as follows: five for grain weight; three each for grains per panicle, panicle length and panicle per plant; and two for grain yield. A QTL for panicle length was consistently detected for both seasons. This QTL caused a significant reduction in the panicle length which explained 10.9% of the total phenotypic variance. For grain yield, two QTL were detected which caused an increase in yield. Two QTL with the highest LOD scores were GPP 7.2 (grains per panicle) in 2009 DS and GW 4.1 (grain weight) in 2010 DS. A total of 32 SSRs with significant effects were identified flanking the QTL distributed across 7 chromosomes. Regions around these SSRs may be further studied to verify the QTL detected.
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