Disease resistance induction in cucumber plant by the treatment with macerated cucumber leaves inoculated with Colletotricum lagenarium, the causal agent of cucumber anthracnose
2007
Negishi, Y. | Yamaguchi, Y., Tokyo Univ. of Agriculture, Tokyo (Japan)
It had been reported that the upper leaves of the cucumber plant that was previously inoculated by Colletotricum lagenarium, the causal agent of cucumber anthracnose,were to be immunized against following inoculation by the same pathogen. The leaves of immunized plant showed no or less anthracnose lesion compared with the non-immunized plant. The inoculated cucumber leaves bearing many lesions were sampled, frozen at -70 deg C and macerated to make sap solution. The cucumber seedling was sprayed by supernatant of the solution, incubated in glass house and sprayed with spore suspension of C. lagenarium on the upper leaves 2 days later. The leaves of the treated seedling showed almost no or very mild symptom while so many lesions were seen on the leaves of the control seedling treated by sterile water. Relatively small number of lesion was seen on the cucumber seedling sprayed with even the supernatant of the healthy cucumber leaf sap solution. The spore of C. lagenarium could germinate and grow evenly both in the supernatant and sterile water but the appresorium seemed to be formed only in sterile water.
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