Gene for actin is a suitable internal reference for relative RT-PCR [reverse transcription-polymerase chain reaction] based expression analysis in normal and mutant 'makapuno' endosperm of coconut(Cocos nucifera L.)
2011
dela Cruz, R.Y., Central Mindanao Univ., Musuan, 8710 Bukidnon (Philippines). Dept. of Biology | Laude, R.P | Diaz, M.G.Q., Philippines Univ. Los Baños, College, Laguna (Philippines). Inst. of Biological Sciences | Laurena, A.C., Philippines Univ. Los Baños, College, Laguna (Philippines). Inst. of Plant Breeding | Mendioro, M.S., Philippines Univ. Los Baños, College, Laguna (Philippines). Inst. of Biological Sciences | Mendoza, E.M.T., Philippines Univ. Los Baños, College, Laguna (Philippines). Inst. of Plant Breeding
Makapuno is an abnormal coconut with overproliferating endosperm that is softer and fluffy due to accumulation of galactomannan. To normalize the expression of the genes possibly involved in the makapuno phenomenon by semi-quantitative polymerase chain reaction(PCR), an internal control gene was determined. The gene encoding actin exhibited a uniform expression in all the samples studied. In contrast, the expression of the genes coding for glyceraldehyde-3-phosphate dehydrogenase(GAPDH) and enolase considerably varied. Therefore, the gene for actin can be used as reference both for normal coconut and mutant makapuno solid endosperm samples at stages 5-6, 6-7 and 7-8 mo.
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