Isolation of banana genes involved in the developmental control of banana ripening
2010
Ocampo, E.T.M., Philippines Univ. Los Baños, College, Laguna (Philippines). Inst. of Plant Breeding. Crop Science Cluster
This study reports on the isolation and characterization of a novel gene with a role in the developmental regulation of banana ripening. The gene, MaMADS2, has a putative protein sequence structurally similar to Type 11 MADS-box transcription factors. MaMADS2 was amplified, isolated and sequenced from ripe fruit cDNA of the local banana cultivars Cavendish, Lakatan, Saba and Señorita. PCR products were sent for sequencing. For confirmation, the products were cloned and re-sequences. The different MaMADS sequences from the different cultivars were compared. A reverse primer from a portion of the K-region of the MaMADS2 gene was designed and used for the preliminary isolation of the DNA region upstream MaMADS2. The promoter of Cavendish was isolated and a putative promoter map was constructed based on preliminary data. Sequences of the promoter regions of the different local cultivars were submitted to PLACE (A Database of Plant cis-Acting Elements) for scanning and identification of known regulatory elements as well as their types and positions in the DNA. Further purification, characterization and comparison with other promoters deemed necessary in understanding the regulatory roles of these promoters. The promoters from the different cultivars contained diverse functional elements. Cavendish, Lakatan and Señorita have very similar cis-elements which clustered on similar sites in the DNA. However, the type and positioning of the elements in Saba were different. Known binding sites for MADS-box genes were present, hinting at possible autoregulation of MaMADS2 gene expression.
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