Control of dirty panicle disease of rice with antagonist microbes under field conditions
2009
Chuaboon, W. | Prathuangwong, S.
Dirty panicle disease caused by multiple fungus species is severe for economic loss of rice production areas in Thailand that the effective control measure is critical limitation. Biological control using microorganism may be necessary for alternative method of sustainable rice production. The two-bacterial biocontrol agents Pseudomonas fluorescens SP007s and Bacillus amyloliquefaciens KPS46 have been proven effective in controlling several diseases of various crops, but their effects on dirty panicle infection of rice are not known. Field experiment was conducted in March-July, 2008 at Angthong growing region to develop control strategies for this disease with seven treatments (T1 to T7) including 4-different antagonist formulations (T1 to T4) compared with 2 chemical programs (T5 and T6) and nontreated control (T7). They were arranged in CRD [complete randomized design] that evaluated under natural inocula. The results showed that 2-treatment program: 6-month shelf life formulation of 1x10sup6 Cfu/ml SP007s (T1:seed treatment and 5 foliar spray intervals) and synthetic fungicide (T6: copper hydroxide seed treatment and 6-carbendazim foliar spray intervals) were significant greatest (P=0.05) for reduced development of dirty panicle infection. Regarding the efficacy of pathogen inhibition, SP007s formulation was significantly higher in suppression of causal species of dirty panicle pathogens. However, all antagonist microbe treatments including SP007s and KPS46 formulations and Trichoderma cell culture except Bacillus subtilis formulation (Laminar) significantly provided higher yields than chemicals T5 and T6 (P=0.05). These antagonist microbes tested seem to enhance biocontrol activity not only with decreased disease severity but also promoted plant growth of rice clump, panicle, and kernel that result in highest yield obtained.
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