High-performance liquid chromatography determination of phenolic acids in potato tubers (Solanum tuberosum) during wound healing.
1992
Ramamurthy M.S. | Maiti B. | Thomas P. | Nair P.M.
The content of phenolic acids formed during wound healing of gamma-irradiated and nonirradiated potato tubers was determined by HPLC. Chlorogenic acid, caffeic acid, p-coumaric acid, and ferulic acid were detected in small quantities in resting whole tubers of irradiated and nonirradiated potatoes. During wound healing their content increased many fold, and in addition, the neo and crypto isomers of chlorogenic acid accumulated in the wound healing tissue. The increased formation of phenolics was accompanied by a parallel rise in phenylalanine ammonia lyase activity. Chlorogenic acid contributed about 56% and together with the neo and crypto isomers accounted for 88% of the phenolics formed. Tubers irradiated to 100 Gy for sprout inhibition showed significantly lower levels of chlorogenic acid and its isomers during the first 8 days of wound healing. The results point to an impairment of wound-induced biosynthesis of phenolics in general and chlorogenic acid and its isomers in particular by irradiation.
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