Cloning of a cellulase gene from indigenous strain of Bacillus species
2011
Zafar, M. (University of Agriculture, Faisalabad (Pakistan). Dept. of Chemistry and Biochemistry) | Sheikh, M.A. (University of Agriculture, Faisalabad (Pakistan). Dept. of Chemistry and Biochemistry) | Sajjad-ur-Rehman (University of Agriculture, Faisalabad (Pakistan). Inst. of Microbiology) | Jamil, A.
The following study presents the cloning of a cellulase gene from indigenous strain of Bacillus sp. The genomic DNA of Bacillus sp was isolated and employed for the PCR amplification of the cellulase gene. For this, different sets of primers were designed following the available sequences of cellulase gene in NCBI data base. A PCR product of cellulase gene of 1.65 Kb was successfully amplified. The amplified gene was cloned into pTZ57R/T cloning vector. The cloning of the gene was confirmed through blue white screening and colony PCR screening.
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