Effect of thidiazuron (TDZ) on in vitro micropropagation of Solanum tuberosum L. cvs. desiree and cardinal
2009
Sajid, Z.A. (Punjab Univ., Lahore (Pakistan)) | Aftab, F. (Punjab Univ., Lahore (Pakistan))
Thidiazuron (1-phenyl-3- (1,2,3- Thiadiazol-5-yl) urea; TDZ) is one of the several substituted ureas that have been investigated recently for their cytokinin-like activity. TDZ is known to be more active than zeatin for stimulating the growth when added to a tissue culture medium at a low concentration. In this study, effect of TDZ on several In vitro growth parameters in Solanum tuberosum cvs. Desiree and Cardinal were observed. Shoot apices (1.0cm each) from both the cultivars were separately inoculated on full strength MS basal media as well as on MS full strength supplemented with different concentrations of TDZ (10(10), 10(9) or 10(8 M) thus forming four combinations. Results were recorded for shoot length, shoot number, root length, root number, number of nodes, fresh and dry weight of plants after 30 days of explant inoculation. MS full strength medium was found to be the best for In vitro micropropagation. However, TDZ did have an influence on the studied growth parameters, which have been discussed and interpreted herein.
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