Occurance of L.monocytogenes in fresh fruits and vegetables from organic farms in west Pomeranian region
2011
Szymczak, B. | Sawicki, W. | Boguslawska-Was, E. | Koronkiewicz, A. | Dabrowski, W,Zachodniopomorski Uniwersytet Technologiczny, Szczecin (Poland). Katedra Mikrobiologii i Biotechnologii Stosowanej
The objective of this paper was to assess the occurrence of L. monocytogenes in fresh fruits and vegetables and to compare different methods of identifying these bacteria. Based on the identification performed according to PN EN ISO 11290-2:2000, Listeria sp was found in 50 percent of beetroot samples, in 25 percent of carrot, in 15 percent of tomatoes and potatoes and in 5 percent of parsley and in 25 percent of strawberries. And when using a multiplex PCR technique, L. monocytogenes was found in 10 percent of the strawberry samples, in 5 percent parsley and 15 percent potatoes. Biochemical analyses should not be the final phase of identifying L. monocytogenes because there are wide discrepancies between the results of this analysis and the results of research conducted using the molecular biology methods. A correlation was shown between the presence of L. monocytogenes in fruits and vegetables and the type of fertilizer used. Listeria sp. and L. monocytogenes were isolated only from the samples of fruits and vegetables grown in farms where soils were manured
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