Phytochemical screening and biological activity of extracts of plant species Halacsya sendtneri (Boiss.) Dörfl.
2012
Mašković, P.Z., Faculty of Agronomy, Čačak (Serbia) | Manojlović, N.T., Medical Faculty, Kragujevac (Serbia) | Mandić, A.I., Institute for Food Technology, Novi Sad (Serbia) | Mišan, A.Č., Institute for Food Technology, Novi Sad (Serbia) | Milovanović, I.Lj., Institute for Food Technology, Novi Sad (Serbia) | Radojković, M.M., Faculty of Technology, Novi Sad (Serbia) | Cvijović, M.S., Faculty of Agronomy, Čačak (Serbia) | Solujić, S.R., Faculty of Science, Kragujevac (Serbia)
This study is aimed at examining total polyphenol, flavonoid, gallotannin and condensed tannins contents in acetone, chloroform, ethyl acetate and petroleum ether extracts of Halacsya sendtneri (Boiss.) Dörfl., their antimicrobial and antioxidant activities, as well as identifying and quantifying the phenolic components. The antioxidant activity is consistent with the results of total quantity of phenolic compound. The results showed that the acetone extract of plant species Halascya sendtneri (Boiss.) Dörfl. possessed the highest antioxidant activity. The IC50 values determined were: 9.45 +/- 1.55 microg/mL for DPPH free radical scavenging activity, 13.46 +/- 1.68 microg/mL for inhibitory activity against lipid peroxidation, 59.11 +/- 0.83 microg/mL for hydroxyl radical scavenging activity and 27.91 +/- 0.88 microg/mL for ferrous ion chelating ability. The antimicrobial activity was tested using broth dilution procedure for determination of the minimum inhibitory concentration (MIC). The MICs were determined for 8 selected indicator strains. All of the extracts showed strong to moderate strong antimicrobial activity. The phenolic composition of Halacsya sendtneri extracts was determined by the HPLC method. The dominant phenolic compound in acetone, chloroform and ethyl acetate extract is rosmarinic acid. Ethyl acetate extract was also abundant in p-hydroxybenzoic acid and ferulic acid. The main compounds in petrol ether extract were chlorogenic acid and quercetin.
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