Epizoological and serological investigation of West Nile fever in central part of Serbia during 2011
2012
Samokovlija, A., Faculty of Veterinary Medicine, Belgrade (Serbia) | Vojinović, D., Scientific Veterinary Institute of Serbia, Belgrade (Serbia) | Rogožarski, D., Veterinary Specialistic Institute Požarevac, Požarevac (Serbia) | Elezović, M., Faculty of Veterinary Medicine, Belgrade (Serbia) | Marić, J., Faculty of Veterinary Medicine, Belgrade (Serbia) | Gligić, A., Institute of Virology, Vaccines and Sera Torlak, Belgrade (Serbia) | Đuričić, B., Faculty of Veterinary Medicine, Belgrade (Serbia)
West Nile Virus (WNV) has been very active in past years in Europe, especially in the surrounding countries of the Republic of Serbia. Constant threat of epidemics has lead to increased level of awareness of whole veterinary and medical services, with emphasis on laboratory investigation of seroprevalence and monitoring of WNV hot spots in nature. In continuation of past investigation on seroprevalence of WNV, total of 1382 blood sera of different animal species and human were obtained and tested for the presence of specific antibodies for WNV, The collected geographically belonged to central part of Serbia. Method of agar gel immunodiffusion test (AGID) was used in our research, followed byconfirmation of positive results by plaque reduction and neutralization test (PRNT-90). The results revealed the presence of specific antibodies for WNV in 1/456 human samples (0.22%), 13/468 dog blood sera (2.78%), 19/235 hors blood sera (8.08%), but specific antibodies for WNV were not found in 10 blood sera of cattle, 102 sheep blood sera, 6 goat blood sera, 2 geese blood sera, 3 duck blood sera, 86 chicken blood sera and 14 pheasant blood sera. The results showed activity of WNV in the Republic of Serbia in 2011, although we did not have confirmed clinical cases of WNV fever in any animal species or humans.
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