Some aspects of implementation [of] DNA markers in animal breeding | Neki aspekti upotrebe DNK markera u oplemenjivanju životinja
2012
Vidović, V., Poljoprivredni fakultet, Novi Sad (Serbia) | Stupar, M., Poljoprivredni fakultet, Novi Sad (Serbia) | Lukač, D., Poljoprivredni fakultet, Novi Sad (Serbia) | Štrbac, Lj., Poljoprivredni fakultet, Novi Sad (Serbia)
DNA-based typing will replace blood group and protein marker typing, although the adoption of a new system will require breeding animals that previously had been typed to be re-tested using DNA markers. Commonly used types of genetic markers are: RFLP (restriction fragment length polymorphism), SSLP (simple sequence length polymorphism), AFLP (amplified fragment length polymorphism), RAPD (random amplification of polymorphic DNA), VNTR (variable number tandem repeats), microsatellite polymorphism, SNP (single nucleotide polymorphism), STR (short tandem repeat), and MS (minisatellites). Aim of this paper is to review some of available DNA markers in horse, sheep, pig and cattle breeding. The current commercially available panel comprises 20 microsatellite horse markers: AHT4, AHT5, ASB2, HMS3, HMS6, HMS7, HTG4, HTG10, VHL20, ASB17, ASB23, LEX33, LEX3, CA425, HMS1, HMS2, HTG6, HTG7, UM011, AME. In sheep is known 528 markers, in pig 1446 and in cattle 3907 markers. Application of DNA-based methods in animal breeding programs offer several advantages over conventional parentage assays in terms of their accuracy and specificity, the ability to use a range of easily obtainable sample tissues including hair, and laboratory analysis with commonly available molecular biological reagents.
显示更多 [+] 显示较少 [-]