Acrylamide triggers hepatic mast cell activity in peripubertal rats
2011
Kovač, R., Faculty of Sciences, Department of Biology and Ecology, Novi Sad (Serbia) | Rajković, V., Faculty of Sciences, Department of Biology and Ecology, Novi Sad (Serbia) | Koledin, I., Faculty of Sciences, Department of Biology and Ecology, Novi Sad (Serbia) | Matavulj, M., Faculty of Sciences, Department of Biology and Ecology, Novi Sad (Serbia)
Acrylamide (AA) as monomer is a potentially both toxic and cancer causing chemical, which can be formed in starchy food heated at 120 deg C temperature or higher. There is public health concern to evaluate whether AA intake from food is an important health risk factor?! The popualtion of nonparenchimal liver cells, such as hepatic mast cells (HMCs) might have a pivotal role in detoxification processes, as well. The aim of the study was to investigate the effects of 21 day-treatment with 25 mg/kg per body weight of AA on HMCs in the juvenile/peripubertal Wistar rats, from their 23rd postnatal day (PND). Liver samples were taken, fixed in formalin, routinely processed for paraffin embedding, cut into 5 micro m thick tissue sections and stained with 0.5% toluidine blue for specific staining of cytoplasmatic secretory granules in HMCs. Qualitative and quantitative evaluations of HMCs were performed by histological, stereological and subsequent statistical analysis on 20 portal triads per animal. The stereological test system M42 was used to evaluate the numerical (Nvm) and volume (Vvm) densities of total, intact and degranulated mast cells. Histological analysis showed an increase in HMCs number and activity in AA-treated group, what was confirmed with statistically significant increase of the Nvm of total and degranulated HMCs. The increase in number and activity of HMCs might be assigned, either to direct activation of HMCs by AA, or indirect via released mediators from hepatocytes to achieve more coordinated and effective AA metabolism. I addition to that, a new question arises: Is AA potentially a new food allergen?
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