Application of DNA (RAPD) and ultrastructure to detect the effect of cadmium stress in Egyptian clover and Sudan grass plantlets
2012
Amina A. Aly
Background In recent years, several plant species have been used as bioindicators to evaluate the toxicity of environmental contaminants on vegetal organisms. In this study, Egyptian clover and Sudan grass seedlings were grown in four cadmium (Cd) concentration levels (0.0, 25, 50 and 100 µM) in MS media to analyze growth responses, Cd accumulation in the shoots and roots of plantlets, proline contents, chlorophylls content and MDA levels of both plantlets. As well as RAPD analysis and leaves ultrastructure were detected. Results The results showed that there was a significant decrease in root and shoot lengths, Chl a, Chl b, total Chl and carotenoids contents for both Egyptian clover and Sudan grass. However, there was a significant increase in Cd accumulation, proline and malondialdehyde (MDA) levels. The genetic variation between Egyptian clover and Sudan grass were evaluated using random amplified polymorphic DNA-polymerase chain reaction (RAPD-PCR) markers to establish specific DNA markers associated with Cd stress. The results of transimssion electron microscopy (TEM) showed a clear disorder in the Cd treated Egyptian clover and Sudan grass seedlings. Conclusion In conclusion, biochemical, molecular and ultrastructure changes in Egyptian clover and Sudan grass could be used as a useful biomarker assay for the detection of genotoxic effects of Cd stress on plants. However, it is necessary to be further confirmed and optimized in the future research.
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