Detection of lasalocid residues in the tissues of broiler chickens by a new screening test total antibiotics
2011
Kožárová, I., University of Veterinary Medicine and Pharmacy in Košice (Slovak Republic) | Šimková, J. | Mártonová, M. | Mačanga, J. | Levkut, M.
The aim of the present study was to evaluate the microbial growth inhibition test Total antibiotics with the test organism Bacillus stearothermophilus var. calidolactis for the screening of lasalocid residues in the tissues of broiler chickens after its oral administration in medicated feed. The residues were investigated throughout the 5-day withdrawal period (WP) and also on day 6 representing the first day following the WP. All broiler chicken tissues were positive for lasalocid. The breast muscle was positive (the presence of residues at-above the detection limit (LOD) of method) up to day 1 of the WP, the thigh muscle, gizzard, heart, skin and fat up to day 3 of the WP and the liver and kidneys up to day 4 of the WP. When evaluating the dubious results (the presence of residues just below the LOD of method), the breast muscle was suspect positive up to day 3 of the WP and the gizzard, skin and fat up to day 4 of the WP. No positive or dubious results were detected on day 5 of the WP. The LOD of Bacillus stearothermophilus var. calidolactis for maduramycin was 500 microg. per l.
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