Characterization of different PCR methods for the identiification of Cryptosporidium spp.
2011
Ravaszová, P., University of Veterinary Medicine and Pharmacy in Košice (Slovak Republic) | Valenčáková, A. | Luptáková, L. | Malčeková, B. | Goldová, M.
Since Cryptosporidium is an important waterborne pathogen that infects the gastrointestinal and respiratory tracts of mammals, including humans, diagnosis of cryptosporidium infections is an important aspects of public health. A number of nucleic acid based methods have been utilized for the identification of the species and for the specific diagnoses of human and animal cryptosporidiosis. Some of these methods are used for the simultaneous identification of various species of Cryptosporidium in clinical samples (multiplex PCR, DNA microarray). Others are used to shorten the time of the assays (Real time PCR with HRM analysis, PCR-RFLP), or to make the assay more specific nested PCR). Nevertheless, all of the above mentioned methods have their pros and cons. In this paper, we introduce some of the recently used methods in molecular biology.
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