[Unstable and repeating genome elements as a molecular markers in flax (Linum usitatissimum L.)] | Nestabilné a opakujúce sa prvky genómu ľanu siateho ako molekulárne markéry

Intergenic DNA analysis are very effective techniques of plant polymorphism mapping because of the unique biological processes of the repetitive sequence of nucleotides and retransposition in their genomes. The species whose genomes are actively responding to the changes of environment and with the widespread use in industry and medicine can be the answer for new and specific requirements of plant breeders and existing of molecular markers is an active device and applicable interconnection of requirements of both phenomena. Intergenic DNA in flax provides opportunities for closer characterization of the diversity of paternity, especially in facilitating the transfer of properties between the lines required. Based on the results provided by the DNA markers relations between 36 genotypes of flax were evaluated by ISSR, IRAP and REMAP techniques using anchored microsatellite primers and primers derived from two different LTR retrotransposons. Summary evaluation of the results of ISSR profiles and constructed dendrograms refers to the two groups of genotypes, which are similar for each used ISSR primer and are occurred in the nearest clusters. In the first case, UPGMA analysis grouped in the case of the each used primer genotypes Pskow II, Hor Nr. 048, Norfolk Princess, PRFGL 93, Flanders and Daehnfeldt Elite 6. The second group of genotypes that occur in clusters together consists of genotypes Renodlat Oljelin, McGregor, Red Wing, Horan, Marina, St Muenchenberger. 8 and Daero. Using techniques working with retrotransposons flax genotypes are grouped into clusters in a manner of relationship based on the procedure for crossing between the genotypes and clearer relationship towards interdependence of landraces with current varieties and lines are added. The analyses of the lines PRFGL 93, Stamm Fa PRFGL 13 and 77 grouped them into one cluster associated with genotypes Rabenslyst La Plata, Pskow II, Super, Flanders and Ilguniliai. Similarly, the landrace Albocoeruleum, Deubgrc 28197 and 28198 were grouped with La Plata 1938, Marina, Norfolk Princess, Lilas and India. These varieties are heterogeneous in their origin, as well as the economic use and pedigree, so it can be assumed that in their genomes retained features of their more ancient ancestors, or conversely, that the lines concentrated in the variability of their genotypes of several varieties, which may have further significance for both theoretical studies of flax genome, and practical in the field of molecular breeding and genetic engineering...